celltrace violet cell proliferation kit, for flow cytometry Search Results


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Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. <t>CFSE</t> dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor
Celltrace Cfse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. <t>CFSE</t> dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor
Celltracks Vr Autoprep Vr System, supplied by CellSearch inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. <t>CFSE</t> dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor
Violet Fluorescent Cell Barcoding Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. <t>CFSE</t> dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor
Ghost Dye Violet 510 Dead Cell Stain Kit, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CellSearch inc celltracks analyzer
Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. <t>CFSE</t> dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor
Celltracks Analyzer, supplied by CellSearch inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. CFSE dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: CD45RA − Foxp3 high regulatory T cells have a negative impact on the clinical outcome of head and neck squamous cell carcinoma

doi: 10.1007/s00262-017-2021-z

Figure Lengend Snippet: Frequency and characteristics of Treg subsets in patients with HNSCC and in those with benign tumors. The gating strategies for Treg subsets in the PBMCs of patients with HNSCC or benign tumors. I: CD45RA+Foxp3low, II: CD45RA−Foxp3low, and III: CD45RA−Foxp3high (a). The expression of CTLA-4, CD25, and CCR4 on Treg subsets in a representative patient with HNSCC (b). Mean fluorescence intensities (MFI) are indicated in the upper right quadrant. CFSE dilutions of proliferated CD4+ T cells were assessed after 72 h of TCR stimulation with or without indicated Treg subsets at a 1:1 ratio (c), and the number of proliferated CD4+ T cells after the co-culture are shown (d). The percentage of Foxp3+ cells in CD4+ T cells (e), CD45RA−Foxp3high Tregs in CD4+ T cells (F), CD45RA+Foxp3low Tregs in CD4+ T cells (g) and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs (h) in PBMCs of patients with HNSCC (n = 46) and benign tumors (n = 23) before treatment. The percentage of CD45RA−Foxp3high Tregs and the ratio of CD45RA−Foxp3high Tregs to CD45RA+Foxp3low Tregs were increased according to clinical stage (i). Statistical comparisons were performed using the parametric unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant, HNSCC head and neck squamous cell carcinoma, CTLA-4 cytotoxic T-lymphocyte antigen 4, CCR CC chemokine receptor, CFSE carboxyfluorescein diacetate succinimidyl ester, TCR T cell receptor

Article Snippet: Enriched CD3 + T cells were labeled with CellTrace CFSE (carboxyfluorescein diacetate succinimidyl ester) Cell Proliferation Kit (Molecular Probes, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Fluorescence, Co-Culture Assay

Journal: Cell Reports

Article Title: Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

doi: 10.1016/j.celrep.2020.107627

Figure Lengend Snippet:

Article Snippet: Rat anti-Mouse CD117-BV421 (c-Kit) (clone 2B8) , BD Biosciences , Cat#562609; RRID: AB_11154585.

Techniques: Recombinant, dsDNA Assay, Sample Prep, Software, Selection